Cell Fusion of Staphylococcus aureusL-Forms

Cell-wall deficiencies in L-forms of Staphylococcus aureus.

Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

Inactivation of vancomycin-induced unstable L-forms of Staphylococcus aureus by horse serum

Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance and proliferate in spite of the loss of cell wall is called an L- form. Unstable L- forms  can convert to normal cells but stble L-forms can not.cell wall - deffective state may be induced spontaneously or through the action of an appropriate agent such as antibiotic interfering with the synthesis of cel...

Two Dimensional Structural Analysis and Expression of a New Staphylococcus aureus Adhesin Based Fusion Protein

Objective(s) Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA)...

Multiple forms of lactate dehydrogenase in Staphylococcus aureus.

Activities for nicotinamide adenine dinucleotide (NAD)-dependent and NAD-independent forms of lactate dehydrogenase (LDH) were measured in cell-free extracts of Staphylococcus aureus strain PS 6 for the d and l isomers of lactate. Data obtained for the NAD-dependent lactate dehydrogenases indicate that oxidation of both isomers of lactate is due to both an l-lactate-specific LDH and a lactate r...